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In vitro storage conditions of Citrus limon (L.) Burm. cv. Novoafonsky were developed in this study. Three explants were used: nodal regenerants, seedlings, and micrografted plants. The results obtained in present investigation confirmed that slow growth maintenance of lemon microplants on MS medium allows to store micro-grafted plants and seedlings for 8 and 12 months, respectively, without subculturing. It was also noted that low temperature ?10 ± 2°C and light intensity of 1,000 lux maintain seedlings till 12 months and micrografted plants till 8 months on MS medium. The decrease of chlorophyll and carotenoids in leaves was observed during the storage of microplants. It was observed that low temperature and low light intensity were better for the preservation of the photosynthetic apparatus and accumulation of photosynthates in the leaves of lemon regenerants, thereby maintaining a high level of their viability. Thus, the best treatment for in vitro conservation was micrografted plants on MS media in temperature ?10 ± 2°C and light intensity of 1,000 lux.

The effect of glutamine, biotin and adenosine diphosphate (ADP) on growth and cultivars micropropagation of three ornamental species was investigated. The addition of 10 -100 mg/l glutamine in culture media significantly increased rate of multiplication in Cordyline fruticosa and 100 mg/l glutamine showed the same effect for Gerbera jamesonii. Addition of glutamine did not show any effect on shoot length, root number and length in all the three species. Addition of 1 -3 mg/l biotin increased shoot length of Gerbera jamesonii but inhibited shoot length of Cordyline fruticosa and decreased root length of Chrysanthemum hybridum and Gerbera jamesonii. Addition of 5.0 mg/l ADP significantly increased multiplication rate of Cordyline fruticosa and 1.0 mg/l ADP showed similar effect for Gerbera jamesonii.

The study was conducted to develop micropropagation and slow-growth conservation protocol for endangered relict plant species Campanula sclerophylla. The best multiplication rate up to 7.2 shoots per explant was obtained on half strength MS supplemented with 3 mg/l BAP and 1 mg/l IAA. The effects of temperature/light conditions, sorbitol and ABA on shoot growth of Campanula sclerophylla were studied. Addition of 3 g/l sorbitol and 5 mg/l ABA inhibited shoot length and multiplication of C. sclerophylla. Explants were stored for 9 months without subculture at 7ºС and light intensity of 23 lux.

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