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Two novel yeast species were isolated during three independent studies of yeasts associated with natural substrates in Brazil and Taiwan. Analysis of the sequences of the D1/D2 domains of the large subunit rRNA gene showed that these novel species belong to the Wickerhamiella clade. The first was isolated from freshwater and a leaf of sugar cane (Saccharum officinarum) in Brazil and from leaves of Wedelia biflora in Taiwan. Described here as Wickerhamiella slavikovae sp. nov., it differs by 56 nucleotide substitutions and 19 gaps in the D1/D2 region of the large subunit rRNA gene from Candida sorbophila, the least divergent species. The second species, named Wickerhamiella goesii sp. nov., was isolated from leaves and the rhizosphere of sugar cane collected in Rio de Janeiro, Brazil. The species differs by 54 nucleotide substitutions and nine gaps in the D1/D2 domains from Candida drosophilae, its least divergent relative. The type strains are Wickerhamiella slavikovae sp. nov. IMUFRJ 52096 T (5CBS 12417 T 5DBVPG 8032 T ) and Wickerhamiella goesii sp. nov. IMUFRJ 52102 T (5CBS 12419 T 5DBVPG 8034 T ).The clade Wickerhamiella consists, at the time of writing, of 26 species that include five species with a sexual state and 21 that may eventually be found to form ascospores (Lachance & Kurtzman, 2011;Barbosa et al., 2012;Badotti et al., 2013). Most of these species are highly specialized nutritionally and ecologically, and some of them have a strong association with floricolous insects and plant materials (Lachance & Kurtzman, 2011;Barbosa et al., 2012). In the course of three independent studies of yeasts associated with natural substrates in Brazil and Taiwan, four unidentified yeast strains were found to have almost identical sequences in the D1/D2 domains of the large subunit rRNA gene. The least divergent known sequence was that of Candida sorbophila. Another four unidentified isolates were recovered from leaves and the rhizosphere of sugar cane (Saccharum officinarum) in Rio de Janeiro, Brazil. These isolates, also in the Wickerhamiella clade, exhibited sequence affinity with Candida drosophilae. In this study, we propose the description of these eight strains as representatives of two novel species, Wickerhamiella slavikovae sp. nov. and Wickerhamiella goesii sp. nov., respectively.Strain UFMG-LD2.04 was isolated from a freshwater sample collected from the Lake de Dentro situated in Ecological State Park of Cantão, state of Tocantins, Brazil, in July 2008. The water sample was filtered on sterile nitrocellulose membranes of 0.45 mm pore size and 47 mm diameter using a Nalgene filtering device. The membrane was placed on the surface of YM agar (0.3 % yeast extract; 0.3 % malt extract; 0.5 % peptone; 1 % glucose; 2 % agar; The GenBank/EMBL/DDBJ accession numbers for the large subunit rRNA gene D1/D2 domain sequences of Wickerhamiella slavikovae IMUFRJ 52096 T and Wickerhamiella goesii IMUFRJ 52102 T are FJ463264 and JN790617, respectively.