Studies were conducted in the I. V. Michurin Federal Scientifi c Center and in its Middle Russian branch. For the fi rst time in the North-Eastern part of the Central black earth region, researches are being conducted to study the microbiota of the soil in the garden of pome crops. The aim of this research was to study the micromycetic composition of the soil in the agrocenosis of a multifactorial experience of an intensive apple orchard. Soil samples were taken in the space between rows and near-stem stripes on the sodding, in the space between rows and near-stem stripes on the fallow land. Experimental conditions: 1) control (natural conditions); 2) optimal moisture (irrigation, soil moisture 80 %); 3) waterlogging (irrigation, soil moisture 120 %). As a result of the conducted research, the dependence of the number of phytopathogenic and saprotrophic populations on the elements of agrotechnology was shown. The maximum number of the population was marked in the near-stem stripes on the fallow land and the minimal number was marked in the near-stem stripes on the sodding. The eff ect of moistening on the number of soil micromycetes was revealed. 80 % moisturized sodded space between rows and near-stem stripes, space between rows on fallow land at a humidity of 120 %, and near-stem stripes with 80 % moisture contained a high number of phytopathogens in relation to natural conditions. The greatest species diversity of the soil mycobiota was observed in the sodded near-stem stripes. A strong correlation was established from reverse close (r = -0.98) to positive close (r = 0.83) in relation to the phytopathogens and saprotrophs in the sodded row spacing; in the soil of the sodded near-stem stripes – from close negative (r = -0.90) to close positive (r = 0.92). It was noted that soil mites did not tolerate the over moistened soil, nematodes, on the contrary, were activated in soils with a humidity of 80 % and 120 % in sodded row spacing. The correlation between nematodes and soil mites in all variants remained strong (r = 0.86-0.97), closer in the control. The number of micromycete organisms in the soil was determined by a modifi ed fl otation method. In the course of research, 1 ml of distilled water was added at one of the stages, instead of a 0.1 % solution of potassium pyrophosphate; on the other, 29 ml of 0.1 % potassium pyrophosphate solution was added instead of 29 ml of distilled water.
Economic losses incurred during raspberry storage are primarily a result of fungal spoilage. This study aimed to characterise the micromycete phytopathogenic complex in stored raspberries. The fungal microbiome was assessed in six Russian raspberry cultivars (Cleopatra, Novost’ Kuzmina, Sulamiph, Fregat, Shakhrazada, Yarkaya) as well as three foreign varieties (Glen Lyon, Cascade Delight, Limonnaya). The economic and biological traits of the cultivars were studied within the “Programme and Protocol for Fruit, Berry and Nut Crops Varietal Testing”. Internal and surfacelevel contamination in infected fruit tissues was detected via the stimulation of microbial growth in a wet chamber. The micromycete species composition was assessed via commonly used methods. The following raspberry spoilage organisms were identified as being predominant. Rots: black (Alternaria alternata (Fr) Keissler), dry (Fusarium Link.), leather (Phytophthora cactorum (Leb. et Cohn) Schroet), grey (Botrytis cinerea Pers.); moulds: black (Cladosporium Link.), green-blue (Penicillium Link.), green-yellow (Aspergillus P. Micheliex Haller), grey capitate (Rhizopus nigricans Ehrenb.), capitate (Mucor spp.); drupe rot (Phragmidium rubi Wint.); bacterioses. Microbiotic profiles varied between cultivars in storage. The fungal genera Fusarium and Alternaria prevailed, with the former dominating in almost all samples. The phytopathogenic complex primarily consisted of the fungal genera Fusarium, Alternaria and Penicillium. Raspberry cultivars which had a weak (Sulamiph, Shakhrazada, Novost’ Kuzmina) or strong (Cleopatra) resistance to micromycetes in storage were identified.
The article presents the results of studies of the influence of dump tillage, fertilizers, on the development of soil pathogens that cause root rot of winter wheat, their parasitic activity is shown harmful mycobiota. The optimal dose of mineral fertilizer was revealed against the background of dump soil cultivation, which made it possible to successfully restrain and increase the number of micromycetes causing root rot
The research was carried out in the Central Russian branch of the Federal Research Center named after I.V. Michurin ”. Soil samples for the study were taken from experimental plots of the Tambov Research Institute of Agriculture of the branch of the Federal State Budgetary Scientific Institution “FNTs im. I.V. Michurin ”. For the first time in the northeastern part of the Central chernozem region, research is being conducted to study the microflora and microfauna of typical chernozem. The aim of the study is the effect of combined treatments, as well as the system of fertilizers on the accumulation, structure of the complex of soil phytopathogenic and saprotrophic mycobiota, as well as the microscopic population of the soil in crops of spring barley. As a result of mycological studies, a complex of soil mycobiota was revealed and the dependence of phytopathogens and saprotrophs on elements of agricultural technology was shown. Dump-surface tillage to one degree or another contributed to stabilization of the antagonistic soil mycobiota of the soil. The optimal dose of mineral fertilizer against the background of combined soil treatments has been determined, which makes it possible to successfully restrain the number of micromycetes that cause root rot. It was noted that micromycetes of the genus Pythium were most often found in soils for all crops, less than other phytopathogens. The research results have proved the peculiarity of identifying structures by the improved method.
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