Introduction and aim. Chemical burns of the skin are common type of injuries both in private life and in industries. Local treatment of chemical burns using wound healing creams and ointments is predominant. Hydrophobic wound healing medicinal products dominate the Ukrainian pharmaceutical market but their hydrophobic base disturbs the healing process of skin. The aim of this work was biochemical and planimetric investigation of treatment efficacy of chemical burns with hydrophilic creams containing ceramides and dexpanthenol. Material and methods. The experiments were performed on 30 rats weighing 190–220 g. In a rat skin burn model, animals were exposed to 9% acetic acid solution. Treatment was initiated after wound appearance and included application of creams containing ceramides and dexpanthenol. The effectiveness of treatment was estimated using planimetric parameters, such as: surface area of necrotic tissue (S, mm 2 ) and cumulative reparative effect. Levels of the biochemical markers such as total protein, creatinine, C-reactive protein (CRP) and content of SH-groups were measured in the rats’ blood serum. Results. It was established that cream developed with ceramides and cream with dexpanthenol exhibits reparative properties at the level of 29 % and 4.5 %, respectively. Biochemical investigations demonstrated the treatment efficacy of creams containing ceramides and dexpanthenol. In terms of CRP level and content of SH-groups, the therapeutic action of cream with dexpanthenol was highly significant by a factor of 1.45 and 1.35, respectively in contrast to the cream with ceramides. Conclusion. Using the chemical burn model and results of planimetric and biochemical research it was found that cream with ceramides and cream with dexpanthenol exhibit wound-healing properties. In-depth study on the wound-healing mechanism of investigated creams with the aim of creating effective hydrophilic creams for use in burn treatment is prospective.

Б а с р е д а к т о р ы х. ғ. д., проф., ҚР ҰҒА академигі М. Ж. Жұрынов Р е д а к ц и я а л қ а с ы:Р.Ш. проф. (Ресей) Абишев М.Е. проф., корр.-мүшесі (Қазақстан) Аврамов К.В. проф. (Украина) Аппель Юрген проф. (Германия) Баймуқанов Д.А. проф., корр.-мүшесі (Қазақстан) Байтулин И.О. проф., академик (Қазақстан) Банас Иозеф проф. (Польша) Берсимбаев Р.И. проф., академик (Қазақстан) Велесько С. проф. (Германия) Велихов Е.П. проф., РҒА академигі (Ресей) Гашимзаде Ф. проф., академик (Əзірбайжан) Гончарук В.В. проф., академик (Украина) Давлетов А.Е. проф., корр.-мүшесі (Қазақстан) Джрбашян Р.Т. проф., академик (Армения) Қалимолдаев М.Н. проф., академик (Қазақстан), бас ред. орынбасары Лаверов Н.П. проф., академик РАН (Россия) Лупашку Ф. проф., корр.-мүшесі (Молдова) Мохд Хасан Селамат проф. (Малайзия) Мырхалықов Ж.У. проф., академик (Қазақстан) Новак Изабелла проф. (Польша) Огарь Н.П. проф., корр.-мүшесі (Қазақстан) Полещук О.Х. проф. (Ресей) Поняев А.И. проф. (Ресей) Сагиян А.С. проф., академик (Армения) Сатубалдин С.С. проф., академик (Қазақстан) Таткеева Г.Г. проф., корр.-мүшесі (Қазақстан) Умбетаев И. проф., академик (Қазақстан) Хрипунов Г.С. проф. (Украина) Юлдашбаев Ю.А. проф., РҒА корр-мүшесі (Ресей) Якубова М.М. проф., академик (Тəжікстан) «Қазақстан Республикасы Ұлттық ғылым академиясының Хабаршысы».

Commonly, the production of herbal medicines is more advantageous economically than chemical synthesis. Agricultural crops, having sufficient raw material base, are of main interest. In this respect the shells of haricot bean (Phaseolus vulgaris L. (Fabaceae)), that are waste products of beans, are of special interest. Due to the absence of regulatory documents for this kind of drug raw material in Ukraine the development of standardization parameters for the raw material of haricot bean is a very topical. Pharmacotherapeutic action of this drug raw material is caused by the presence of amino acids, proteins, macro- and microelements as well as flavonoids. There are medicines at the pharmaceutical market of Ukraine that was produced from the haricot bean: «Sadifit» (ZAO «Liktravy», Ukraine), «Arphazetin» (ZAO Pharmaceutical plant «Viola», Ukraine), «Hepatophyt» (LLC «Research and production pharmaceutical company «AIM», Ukraine), «Shells of haricot bean» (ZAO «Liktravy», Ukraine). Medicines are introduced to the market in the form of medicinal herbs mixture that's why main method of application is a preparation of restorative drinks, tinctures, teas. In light of this, study of amino acid composition in the water extracts from raw material is reasonable. Research of qualitative and quantitative amino acid composition of 5 lots of the shells of haricot bean was carried out by HPLC method. This raw material was harvested in different regions of Ukraine over a period of 2014 – 2016. 16 amino acids were identified in the water extracts of the shells of haricot bean. Glutamic acid (1.60 – 5.56 ´µg/100 mg), L-alanine (1.41 – 2.23 µg/100 mg), L-arginine (0.65 – 2.12 µg/100 mg) had the greatest content in the investigated samples. D-serine (0.04 – 0.13µg/100 mg) and D,L-methionine (0.02 – 0.43 µg/100 mg) had the lowest quantity in the investigated samples. Sufficiently high content of amino acids makes it possible to consider them as potential substances-markers at the development of assay procedure for the standardization of raw material.