Hypercholesterolemia is the leading cause for the development of various diseases made pharmaceutical companies to turn towards the herbal products with fewer side effects. In the present research, the hypocholesterolemic activity of Enicostemma axillare (EA) along with their phytochemical evaluation has been done. Preliminary phytochemical analysis was carried out in different extracts of EA. The hypocholesterolemic effect of 85 % methanolic extract of EA was evaluated in fructose induced hyperlipidemic animals. Antioxidant enzymes such as catalase, TBARS, GSH, GST and lipid profile such as cholesterol, LDL, VLDL, HDL and Triglycerides were analyzed in heart and plasma samples. Administration of EA decreases the lipid profile and TBARS significantly (p<0.05). Likewise, EA administration increases the antioxidant and HDL significantly (p<0.05). The results reveal that EA is a rich source for phytoconstituents and can be used as a potent hypocholesterolemic and antioxidant agent in pharmaceutical industry.

The present study attempts to evaluate the phytochemical activity and in vitro anticancer activity of Eupatorium triplinerve methanol extract against lung cancer cell line A-549 and normal cell line Vero. The compound was analyzed for the presence of various phytoconstituents and screened for its cytotoxicity at five different concentrations by MTT assay. The phytochemical profile exhibited the presence of alkaloids, phenolics, flavonoids and steroids. The study documented that the percentage of cell viability has been reduced with the increased concentration, as evidenced by cell death. Eupatorium triplinerve extract shows potential cytotoxic activity with CTC 50 of 450 µg/ml and 110 µg/ml against A-549 and Vero cell line respectively. The result obtained was comparably potent, the crude extract showed moderate activity against cancer cells and it was less toxic to the normal cells. In future, research need to be explored in the purification of this extract and identification of lead compound which could be used as a therapeutic tool against lung cancer.