Background: H. pylori is a microaerophilic Gram-negative bacterium, spiral in shape. It infects approximately half of the population across the world. Aims: to assess the antibiotic resistance of H. pylori as well as the potential of medical plant extracts to inhibit resistant strains. Materials and Methods: The study was conducted in Microbiology Institute, Shah Abdul Latif University, Khairpur Mir’s from January 2019 to December 2019. Endoscopy was used to obtain samples from the gastrointestinal ward of teaching hospital of KMC, Khairpur. H. pylori was isolated, identified and inoculated. From the local market, ginger roots, garlic roots, kalonji seeds, and mint leaves were acquired and extracts were prepared. RESULTS: Antimicrobial sensitivity of extracts (Garlic, ginger, kalonji and mint) against H. pylori was tested by agar well-diffusion method. All herbal extracts showed more sensitivity extracted in ethanol as compared to distilled water. In contrast mint did not give any results. Ethanol extracts are found to be very effective against H. pylori as compared to distilled water extracts. Conclusion: Garlic, ginger, and kalonji all demonstrated antibacterial property towards H. pylori. Keywords: Helicobacter pylori, Antimicrobial Sensitivity, Plant Extracts, Inhibition Zone