Background: The Bombay phenotype is an uncommon blood group identified using forward and reverses plasma categorizing. Individuals with the Bombay phenotype lack the antigens A, B, and H on their blood cells, but their serum has high levels of anti-A, anti-B, and anti-H antibodies. The Bombay phenotype needed donation of the same blood as the Bombay phenotype or homologous blood. As a result, it is critical to do blood grouping appropriately. Objective: The objective of this is to determine the prevalence of Bombay phenotype among the O blood group donors and patients in Peshawar Khyber Pakhtunkhwa Material and methods: This research was done at Khyber Medical University Peshawar's Institute of Paramedical Science. This trial lasted six months. The samples came from Peshawar's transfusion centres and hospitals. A total of 1050 O blood donors and patients were tested. The tube technique was used to categorise forward and backward blood. Forward blood grouping used anti-sera A, B, and D, whereas reverse blood grouping used known as red blood cells from A, B, and O blood types. Results: No cases of Bombay phenotype were detected in the entire study. Conclusion: According to the findings of this research, the Bombay phenotype is not prevalent in Peshawar. Keywords: Bombay blood group, O blood group donors and patients, forward and Reverse blood grouping.