Introduction: Factors such as comorbidity, age and gender distribution are mostly related to hospitalization, numbers requiring intensive care and case fatality rate. In this review, the fatality rate of coronavirus disease 2019 (COVID-19) in different population health background according to comorbidity, age, gender distribution, and laboratory prognosis for COVID-19. Methodology: The current review was based on the data from copious studies that had homogeneity in relation to the review’s objectives. It included the newest studies from December 2019 to September 2020. The epidemiological reasons for the high morbidity and mortality rates among COVID-19 patients were analyzed in different countries. Results: The highest comorbidity prevalence of COVID-19 was recorded in the United States of America (USA) (93.9%) and Italy (68%). Among population health background factors, comorbidity was the most common cause of COVID-19 fatality in the USA. The mean age of the most COVID-19 fatalities was more than 60 years old. Most of the studies show that 60% of COVID-19 patients were male. The fatality rates for the age group of 80-89 years-old in Korea, China, and Italy were 8.7 %, 14.7 %, and 18.8 % respectively. Lymphocytopenia has been observed in 91% of COVID-19 death cases. C - reactive protein had increased in 40-60% of COVID-19 patients. Conclusions: Many factors contribute to COVID-19 severity and fatality rates. Comorbidity, age, and gender were the main reasons for the Case Fatality Rate. This review recommends to follow preventive measures for overcoming the challenges faced during this emerging pandemic disease.

<b><i>Introduction:</i></b> SARS-CoV-2 is a new type of coronavirus causing a pandemic severe acute respiratory syndrome (SARS-2). Coronaviruses are very diverting genetically and mutate so often periodically. The natural selection of viral mutations may cause host infection selectivity and infectivity. <b><i>Methods:</i></b> This study was aimed to indicate the diversity between human and animal coronaviruses through finding the rate of mutation in each of the spike, nucleocapsid, envelope, and membrane proteins. <b><i>Results:</i></b> The mutation rate is abundant in all 4 structural proteins. The most number of statistically significant amino acid mutations were found in spike receptor-binding domain (RBD) which may be because it is responsible for a corresponding receptor binding in a broad range of hosts and host selectivity to infect. Among 17 previously known amino acids which are important for binding of spike to angiotensin-converting enzyme 2 (ACE2) receptor, all of them are conservative among human coronaviruses, but only 3 of them significantly are mutated in animal coronaviruses. A single amino acid aspartate-454, that causes dissociation of the RBD of the spike and ACE2, and F486 which gives the strength of binding with ACE2 remain intact in all coronaviruses. <b><i>Discussion/Conclusion:</i></b> Observations of this study provided evidence of the genetic diversity and rapid evolution of SARS-CoV-2 as well as other human and animal coronaviruses.

Today, the emerging of the new coronavirus 2019nCoV possesses a global health problem and little is known about its origin. In the current investigation, an evolutionary and molecular epidemiological analysis have provided of this new emerged virus. The phylogenetic trees for animal coronaviruses with the novel coronavirus-2019 have been created using a number of available complete protein sequences of envelope (E), membrane (M), nucleocapsid (N) and spike (S) proteins. The phylogenetic trees analysis illustrated that 2019nCoV in all four proteins are very closely related with coronaviruses isolated from Pangolin (scaly anteater) and Bat-SARS-like-coronavirses because all of them are clustered in the same clade. Whereas, the 2019nCoV is less closely related to coronavirses isolated from Rousettus bat (fruit bat) and MERS coronaviruses isolated from camel because they are gathered in the same clade only in two of the four studied proteins, nucleocapsid (N) and spike (S). In the conclusion, the new 2019nCoV is more likely to be originated from Bat-SARS-like-coronaviruses or/and coronavirus isolated from Pangolin after adaptation and evolution in the human hosts. Because of the number of infected cases to date indicates a very quick human-to-human transmission. Thus, necessitates a very rapid active surveillance using accurate method to find the original host where the 2019nCoV emerged. This will help in further understanding and creating a better approach to control the spread of SARS-CoV-2 outbreak.

Ionizing radiation has an effect on health and genetics and its recently used as therapy for different types of cancer. The study aimed to investigate the effect of therapeutic radiation on the tumor suppressor gene, TP53Three regions of TP53 were investigated, exon-7, intron-7 and exon-8. A total of 77 cancer patients who had radiation therapy were examined in this study for genetic analysis with 80 healthy volunteers (control). Genetic testing was carried out before and after radiation treatment. Three ml of blood sample was taken from each patient for the purpose of DNA extraction. In blood cell DNA, TP53 was PCR amplified and sequenced to check any mutation which may occur after radiation. The result demonstrated short-term radiation there was no significant mutation in TP53 gene after radiation therapy. However, there was a novel mutation indicated in the area of the study, in intron-7 region of TP53 gene, C14166T. This mutation was for the first time observed in the study area and in the world. The mutation exists in both health control (26.6%) and cancer patients (73.3%). The female gender experienced a higher rate (60% ) of this mutation than male at the rate and it's more common in breast cancer patients, 33.3%. It is observed in patients older than 40 at 66.6%. Short-term radiation therapy may not cause a serious gene mutation in patients undergoing radiotherapy. A novel mutation in TP53 exists in the study area which is more common in cancer patients. This novel mutation is higher in females and in old ages. This single mutation might be useful in the future to be used as a diagnostic marker and this needs more study to investigate.

Introduction: Although Cysticercus tenuicollis is one of the most economic and veterinary important parasite in Iraq, scanty molecular characterization exists for this helminth. This study aimed to determine the prevalence and molecular description of C. tenuicollis isolates from sheep in Kalar district of Iraq. Methodology: A total of 2,906 slaughtered sheep were examined post-mortem. Up to 20 samples of C. tenuicollis was extracted and amplified using mitochondrial COX1 gene. Results: The overall prevalence rate was 6.88%, and female sheep recorded higher rate of infection (24.35%) than male (6.16%) with significant difference (p<0.05). The molecular results showed 14 haplotypes for COX1 gene and the pairwise nucleotide variation among them was ranged from 0.2 to 2.6%. Twelve out of fourteen haplotypes of C. tenuicollis involving one to three base mutations were discovered in Kalar, Iraq for the first time and this could be a unique mutation internationally and did not registered previously. Eleven newly recorded haplotypes involved only one single mutation and the remaining one involved three mutations. Phylogenetic interpretation showed that Cysticercus tenuicollis-Kalar isolate were clustered in one clade, and closely related to isolates discovered in Nigeria, China, Turkey, Poland, and Iran. Conclusions: This study provided a new record data on prevalence and discovered novel strains of C. tenuicollis in the study area for the first time named Cysticercus tenuicollis-Kalar isolate. Novel haplotypes might consider endemic genetic characterization of this metacestode. The present data may be useful to provide a good molecular background for future preventive and control programs.