Aim of the study: Determination of liver enzyme [AST(GOT),ALT(GPT),ALK] and evaluate the performance of defferent JAK2 V617F mutation PCR:PCR-ARMS,PCR-RFLP,Real Time-PCR .The patients and methods of work collected samples from the Center for Hematology and Oncology in Kirkuk City , the study included ( 88) patients whose ages ranged between (2-79) and also included ( 40) of natural persons as control cases whose ages ranged between (7-63). In molecular tests, ( 75) a patient sample of the total patient samples was used. The Determination of liver enzyme (AST,ALT,ALK) was performed using several equipment provided by the company (randoxs and Biomerieux) for the studied enzyme, Real-time examination was done using a kit equipped by (Bioneer-Korea) company. Rresults: the results showed the increase in liver enzymes in patients (male and female) compared to (male and female) control at the level of significance. As for the results of molecular tests, the results showed that using the assay (RFLP) that 22 samples gave genotype (GG) and 53 samples gave genotype (GT), as for the assay (ARMS), all samples gave the genotype (GT) Conclusions :High liver enzymes in leukemia patients.

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