Background: <i>Pausinystalia yohimbe</i> (L.) is a member of the family Rubiaceae. It is a valuable medicinal tree, distributed in evergreen closed-canopy forests in West Africa. It is traditionally used for treatment of erectile dysfunction and diabetes. Objective: This was an attempt to evaluate the effects of methanolic extract of <i>P. yohimbe</i> bark on blood glucose level in normal fasting rats. Methods: Different doses of methanolic extract of <i>P. yohimbe</i> bark (5, 10, 20, 40 and 80 mg/kg/mL) were orally administered to normal fasting rats to assess their effects on blood glucose levels. Results: The methanolic extract of P. yohimbe bark in different concentrations (5, 10, 20, 40 and 80 mg/kg/mL) when administered to normal fasting rats, only a considerable reduction (26.57 mg/dL) was produced by the dose of 20 mg/kg/mL. Conclusion and Recommendation: Although <i>P. yohimbe</i> has long been reported to regulate blood glucose levels; such effect is unclear and remains requiring further studies