The effect of isomalto-oligosaccharides on human fecal flora was studied. Bifidobacteria and the Bacteroides fragilis group from human intestine could utilize isomalto-oligosaccharides but Escherichia coli and other bacteria could not. After the administration of isomaltooligosaccharides (13.5 g daily for 2 weeks) to healthy adult men and senile persons, the numbers of bifidobacteria in the feces increased. The consistency of feces was improved. These studies may support the usefulness of isomalto-oligosaccharides as components of new healthy foodstuffs for the improvement of the intestinal flora.

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Isomaltooligosaccharides (IMO), sweeteners derived from corn-starch, selectively promote the growth of bifidobacteria in the human intestine. The metabolic fate of IMO in healthy men was investigated. The expiration rates of excess (13)CO2 and hydrogen of six men were measured while sedentary and while taking physical exercise after the (13)C-labeled IMO intakes. The breath H2 excretion kept at a constant state after IMO ingestion in the sedentary test and increased in the exercise test. The serum glucose and serum insulin increased 30 min after IMO ingestion. The (13)CO2 recoveries were 28.7% in the sedentary test and 60.9% in the exercise test. These recoveries were 70-80% compared those of maltose. These results indicated that a part of IMO was digested and the residual IMO was fermented by intestinal flora. The energy value of IMO might be about 75% of that of maltose.

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Isomaltooligosaccharides have a 1 ->6 glucosidic linkages such as isomaltose, panose and isomaltotriose. Wehave developed a new sugar, Isomalto-900R,1) which is a mixture of isomaltooligosaccharides.The effect of isomaltooligosaccharides on human fecal flora was studied. After administering isomaltooligosaccharides (13.5 g daily for 2 weeks) to healthy adult men and senile persons, the number of bifidobacterial cells in the feces increased.2) In this report, we determined the minimumdosage of isomaltooligosaccharides for the proliferation of bifidobacteria. Isomalto-900R was prepared from corn starch by the actions of a-amylase, pullulanase and a-glucosidase.Isomalto-900R contains 25% water, and on a dry basis, was composed of 85.4% isomaltooligosaccharides, 10.5% maltose and maltotriose, and 4.1% glucose. The isomaltooligosaccharides included 37.2% di-, 26.8% tri-, and 21.4% above tetra-saccharides.