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The metabolic responses of eucal ptus and safflower cells to hydrodynamic stress in a 550-cm3 stirred tank reactor larger than the decrease in the membrane integrity. Intracellular NAD(P)H was decreased and cytosolic calcium content was increased by agitation. EGTA, verapamil and W-7 suppressed the decrease in NAD(P)H. These results suggest that the cytosolic calcium, as a second messenger, mediate the signal of the hydrodynamic stress into the metabolism which induces the decrease in respiration rate. Furthermore, a decrease in the filamentous actin content due to agitation was observed.This work was presented at the 46th annual meeting of the Can. SOC.

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