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Working with the suprarenal vein blood, Sugawara reported, some years ago, from our Laboratory of the existence of a certain dif ference between the values of epinephrine therein determinable by two kinds of method, that achieved by the cat paradoxical pupil reaction being inferior to that arrived at by the rabbit intestine segment me thod. The mean of the ratio in 34 observations was noted as 1:1.7.1) The suprarenal extracts made from several kinds of animals were also estimated by the biological methods above mentioned, and the colori metrical method of Folin, Cannon and Denis. Kodama's modifi cation found its application. The values yielded by the three methods did not coincide with each other, but there existed certain ratios be tween them.2)Simultaneously with the second work of Sugawara, several investigations attacking the same line of thought and extending in several directions were started. The present investigation involving a large number of the methods and a large number of different ani mals was one of them, and the other papers have been reported prior to this. The latter involve the following data which will be recapitu lated in their order of appearing in this Journal.According to Kojima and Saito, the bovine suprarenal medulla extracted by Folin gives to the method of Folin and others and the 1) T. Sugawara, Tohoku J.

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