Vitis vinifera (V. vinifera) is a herbaceous plant, cultivated worldwide and known for its biological benefits. The aim of this study is the investigation of the chemical composition as well as the determination of the biological potential of different grape stem extracts obtained by maceration and accelerated solvent extraction (ASE). The HPLC analysis of the tested extracts led to the identification of 28 compounds of which 17 were identified for the first time in grape plants, in addition to seven revealed in the stem part for the first time. Twenty-nine volatile molecules have been detected by GC-MS in the grape stem part; among them seven were identified for the first time in the grape plant. For the biological analysis, the ethyl acetate extract (EtOAc) obtained by maceration showed a significant potential regarding antioxidant activity (IC50 = 42.5 µg/mL), anti-Alzheimer (IC50 = 14.1 µg/mL), antidiabetic (IC50 = 13.4 µg/mL), cytotoxic with HCT-116 (IC50 = 12.5 µg/mL), and anti-inflammatory (IC50 = 26.6 µg/mL) activities, as well as showing the highest polyphenol content (207.9 mg GAE/g DW).

Phytochemical properties have recently increased the popularity of plant polysaccharides as wound dressing materials. This work aims at studying the structural characteristics of polysaccharides extracted from Moringa leaves (Moringa Leaves Water Soluble Polysaccharide: MLWSP), and its antioxidant activities, cytotoxic effects, and laser burn wound healing effects in rats. This MLWSP was structurally characterized. Results showed 175.21 KDa and 18.6%, respectively, for the molecular weight and the yield of the novel extracted polysaccharide. It is a hetero-polysaccharide containing arabinose, rhamnose, and galactose. XRD suggested a semi-crystalline structure of the studied polymer and FT-IR results revealed a typical polysaccharide structure. It is composed of 50 to 500 µm rocky-shaped units with rough surfaces and it was found to inhibit the proliferation of the human colon (HCT-116) (IC50 = 36 ± 2.5 µg/mL), breast (MCF-7) (IC50 = 48 ± 3.2), and ovary cancers (IC50 = 24 ± 8.1). The MLWSP showed significant antioxidant effects compared to Trolox (CI50 = 0.001 mg/g). Moreover, promising wound healing results were displayed. The effect of MLWSP hydrogel application on laser burn injuries stimulated wound contraction, re-epithelization, and remodeling phases 8 days after treatment. The wound healing potential of MLWSP may be due to its significant antioxidant activity and/or the huge amount of monosaccharide molecules.