It may be concluded that dysbiosis of GI microflora influences on a degree of dental hard tissue demineralization, which in turn may predispose to the formation of dental caries.

The alkaloid accumulation dymanics in Veratrum lobelianum were investigated. Jervine was found from 0.02 to 0.11% in the subterranean part at all vegetation stages. The jervine content was greatest in subterranean plant organs, reaching a maximum (0.3%) during natural dying off of aerial organs. Jervine, a natural analog of serotonin, can be used as a specific fibroblast growth factor.Veratrum lobelianum growing in various regions of Georgia has yielded 12 pure alkaloids. One of the main alkaloids is jervine [1][2][3][4]. Jervine exhibited activity in screening experiments in a test system of isolated organs sensitive to serotonin.We studied the qualitative and quantitative composition of the total alkaloids and the jervine content in various plant organs by vegetative phases in order to find the optimal collection times of the raw material and to isolate biologically active compounds from V. lobelianum. According to our results, total alkaloids in V. lobelianum varied as functions of development phase and plant habitat [1,3].The jervine accumulation dynamics in aerial organs of V. lobelianum (Table 1) showed that the alkaloid is present in practically all stages of active vegetation. The content was maximum at a plant height of 20-30 cm. The variability of the alkaloid content separately in stems and leaves showed that jervine accumulated more in leaves at the early stage of plant development whereas the content was higher in stems than in leaves at the start of flowering. The situation changed during fruiting. The amount of jervine decreased in stems and increased in leaves although it remained less than at the start of vegetation.Pseudojervine appeared in the total alkaloids slightly later after the plant height reaches 20-30 cm. Veralosine dominated compounds containing the 22,26-iminocholestane skeleton among total alkaloids in subterranean organs of V. lobelianum in the early vegetative stages. The veralosidine and veralosinine contents remained practically constant from the appearance of young shoots until the plant reached a height of 20-30 cm.A spot for rubijervine was observed by TLC in glucoalkaloid fractions from V. lobelianum that attained a height of 20-30 cm.The variability of jervine, pseudojervine, and rubijervine contents in rhizomes with roots showed that the jervine concentration gradually increased from the moment of appearance of aerial organs and reached a maximum during natural dying off of aerial organs whereas the accumulation of pseudojervine was maximum at the start of vegetation and then gradually decreased and reached a minimum when aerial organs start to die off. The rubijervine content remained practically constant until flowering, decreased slightly during flowering, and decreased toward the end of vegetation.The presence of verazine was observed chromatographically in roots at all vegetative stages and in the aerial part of plants from 3 to 30 cm high.The content of total alkaloids in rhizomes with roots of V. lobelianum showed that the amount of alkamines increased from the very...

As a result of the continuing changes in the condition of tissues and organs of the dental cavity and the body as a whole, there is a rise in the intensity of tooth decay and periodontal diseases with the onset of pregnancy. The aim of our research was to look into the prevalence and severity of pathology in the hard tissues of teeth and periodontal tissues during pregnancy, as well as to figure out how to better organize therapeutic and prophylactic steps for pregnant women. A comprehensive epidemiological study of 170 pregnant women aged 18 to 40 living in Tbilisi was carried out according to the WHO method. The findings show a high prevalence of dental cavity (from 61 percent to 98.8%) and a high severity of dental caries among pregnant women (from 2.55 1.03 to 14.31 5.44). The intensity of caries indicators increased as the gestational stage lengthened, but the greatest increase was observed in the second trimester of pregnancy. The results indicate a substantial prevalence of periodontal pathology (according to the PMA index) among pregnant women, with the level of pathology increasing with their age and gestational age (from 41.7 percent to 78.8 percent). By the end of the second trimester of pregnancy, the most noticeable improvements in periodontal tissues had been observed. Oral hygiene played a big role in determining the presence and incidence of periodontal disease. Low dental health indicators and worsening dental status during pregnancy point to the need for a more comprehensive diagnosis of all risk factors for major dental diseases, as well as the implementation of all stages of therapeutic and prophylactic interventions during pregnancy, based on the findings of a complete dental examination.

The research objects were underground and over ground parts of Delphinium speciosum Bieb and Delphinium flexuosum Bieb., which were collected in the Tsikisjvari, Georgia in different vegetation phases. According to the results of the study pharmacological active methyllycaconitine and lycoctonine are dominant basics of the total alkaloids in the underground and overground parts of Delphinium speciosum and Delphinium flexuosum, growing in Georgia.Based on the research it was suggested that the most reasonable period for collection is flowering phase for the overground parts and fructification phase for the underground parts of the above-mentioned species of Delphinium speciosum Bieb. and Delphinium flexuosum Bieb.