Key words:G6G, Ovsynch, presynchronization, fertility, dairy cow.Our objective in this study the effect of parity and average milk production on the conception rate after resynchronization with G6G protocol or Ovsynch protocol. Lactating Holstein cows (n = 100) divided into two groups (Ovsynch and G6G) cows in this group1 (n = 50) were received the following treatment sequence, 12 µg Buserelin on day zero, 500 µg IM of Cloprostenol on day seven, 12 µg Buserelin IM on day nine and received timed artificial insemination (TAI) 16 hours later. Group II (G6G): were received 500 µg IM Cloprostenol on day zero, 12 µg Buserelin on day two and six days later these cows received the Ovsynch as previously explained in the first group. Conception rate was (40 vs. 30 %) for G6G and Ovsynch protocol respectively primiparous cows, conception rate to G6G program was 46.15%, compared to 28.57% in cows synchronized with Ovsynch program. In Multiparous cows, the similar trend was observed but the difference was smaller (conception rate 37.8 vs. 30.55% in cows synchronized with G6G and with Ovsynch, respectively). In high producers, conception rate was numerically higher in the Ovsynch group (40%) compared to the G6G group (36%). While, in low producers, conception rate to G6G protocol (48%) tended (P = 0.07) to be higher than that to Ovsynch protocol (20%) in conclusion Administration of PGF2α 8 d before initiation of Ovsynch increase the conception rate compared with the standard Ovsynch protocol in primiparous cows than in multiparous cows and in low producers than high producer cows

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The aim of the current study was to compare between different regimens used for treatment of endometritis in mares with the help of ultrasonography. This study was carried out on 50 repeat breeding mares (35 mares with endometritis and 15 mares (control group) without endometritis). All mares were examined by trans-rectal ultrasonography and uterine swabs were obtained for laboratory diagnosis and sensitivity testing. Mares with endometritis were divided into three groups according the type of treatment; group (1) contains 12 mares were treated by I/V infusion of Enrofloxacin once daily for 5 days and I/M injection of 25 IU oxytocin, group (2) contains 12 mares were treated by uterine wash with normal saline for 3 days and I/M injection of 25 IU oxytocin, group (3) contains 11 mares were treated by uterine wash with normal saline and Gentamicin for 3 days and I/M injection of 25 IU oxytocin. The results revealed that uterine horn diameter was significantly larger (p < 0.05) in repeat breeding mares with endometritis (7.28 cm) than that in control (5.18 cm). The best recovery rate was obtained after using Enrofloxacin for 5 days with oxytocin, where (8/12, 66.7 %) mares became pregnant, followed by uterine wash with saline and Gentamicin for 3 days and I/M injection of 25 IU oxytocin, where (6/11, 54.5 %) mares became pregnant and finally uterine wash with saline for 3 days and I/M injection of 25 IU oxytocin, where (4/12, 33.3 %) mares became pregnant. Moreover, ultrasonography is an essential tool for diagnosis of endometritis in mares, Enrofloxacin together with uterine ecbolic as a treatment improved pregnancy rate of affected mares and Enrofloxacin may be an appropriate option for treatment of infectious endometritis associated with susceptible bacterial infection in mares.

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