Chemolithoautotrophy is a primordial process, where chemical energy converts inorganic carbon to organic. The prevalence of chemosynthesis was examined in the mangrove clam, Polymesoda erosa, and the ambient sediment at Chorao Island, Mandovi estuary, Goa. The sediment system is reducing, organically rich, high in electron donors, acceptors, and inorganic carbon. This clam thrives by immersing 75-90% of its body in sulfidic sediments. Hence, it is hypothesized that it could have an adaptive mechanism like microbially mediated utilization of reduced sulfur compound, S2O32- (model compound) coupled to inorganic carbon uptake. During spawning, maximum carbonate uptake rates of 449 and 594 nmole C g dry wt-1 h-1 were recorded in the gill and foot, respectively. Next generation sequencing revealed that Thiothrix and other sulfur oxidizers gathered from ambient sediment were present in gill, mantle and foot in the ratio 1:3:14 and 1:5:6, respectively. It is inferred that the clam and these associated bacteria could make an important contribution to chemosynthetic carbon fixation. The process could serve as an essential supplementary nutritional source especially during the physiologically feeble spawning phase. To the best of our knowledge, this is the first report of chemolithoautotrophic process in P. erosa of the family Cyrenidae.