In the pathogenesis of autoimmune diseases, including immune thrombocytopenia (ITP), maintenance of inflammation and the loss of immune tolerance is essential. Transforming growth factor-beta (TGFβ) is a regulatory cytokine with pleiotropic function. TGF-β inhibits differentiation of T helper (Th1, Th2) cells and promotes the development of peripheral Tregs. The aim of our study was to explore the plasma levels of TGF-β and to perform its correlative analyses with peripheral blood biomarkers of systemic inflammation (NLR, PLR, PMR, SII, dNLR) in patients with ITP who did not respond to the first line treatment and had splenectomy as a second line therapy. The concentrations of TGF-β in plasma was quantified using ELISA kits by eBioscience. Statistical analyses was performed using Graph Pad Prism -Mann Whitney U test; for the correlation analyses we used spearman rank correlation by SPSS program. Our study revealed that the level of TGF-β in patients with refractory ITP is significantly diminished compared to the healthy subjects or patients undergoing splenectomy for other reasons than autoimmune or malignant hematological pathologies. There was no correlation observed between the plasma concentrations of TGF-β and the peripheral blood biomarkers of systemic inflammation.

River Adjaristskhali gorge alongside territories are refugium for third moisture Kolkhetian relictic forest eco-systems which was given UNESCO world natural heritage status. At present construction and exploitation of cascade type derivative hydroelectric stations in the gorge creates risk for fragmentation, destroy and losing of natural habitats. Elemental analysis of underground spring water by plasma atomic-emission spectrometry was carried out at Shuakhevi township location.The obtained data were compared with the results of the multi-elemental analysis of the Achariskali River.The basic macro-elements in spring water and river Adjaristskhali are Ca and Mg. in samples of river Adjaristskhali concentrations of P, Fe, Al are beyond MPC which is the verification for their anthropogenic pollutions. Besides such dangerous pollutants as Hg, Li, Pb, Ti is beyond MPC.