44The lifetime of G. biloba is very long, and its growth is relatively slow. However, 45 little is known about growth-related genes in this species. We combined mRNA 46 sequencing (RNA-Seq) with bulked segregant analysis (BSA) to fine map significant 47 agronomic trait genes by developing polymorphism molecular markers at the 48 transcriptome level. RNA-Seq data provides BSA with genotype information in RNA 49 Pool to screen out linked genes (low in false positives) after data analysis, and the 50 efficiency of development and verification of the linked polymorphism marker is 51 greatly improved. This combined approach (named BSR) has been applied to plant 52 transcriptome sequencing in sunflower, corn, wheat, and Arabidopsis thaliana. In this 53 study, transcriptome sequencing of high growth (GD) and low growth (BD) samples 54 of G. biloba half-sib families was performed. After assembling the clean reads, 601 55 differential expression genes were detected and 513 of them were assigned functional 56 annotations. Single nucleotide polymorphism (SNP) analysis identified SNPs 57 associated with 119 genes in the GD and BD groups; 58 of these genes were 58annotated. This study provides molecular level data that could be used for seed 59 selection of high growth G.biloba half-sib families for future breeding programs. 60