In order to investigate the relationship between inflammation and lncRNA in mixed dry eye disease (DED), this study establishes competitive endogenous RNA (ceRNA) network in mixed DED. Microarray analysis of cornea from mixed DED mice is performed to screen for differences in lncRNA and target genes, and miRNA bioinformatics were predicted based on the ceRNA hypothesis. The ceRNA network, which consists of 96 relationship pairs, is constructed using the top 10 upregulated lncRNAs and all upregulated mRNAs and two pairs of lncRNA-miRNA-mRNA pairs (NONMMUT047964.2-miR-671-5p-Egr-1andNONMMUT054540.2-miR-1934-5p-Grm2) are selected for RT-qRCR verification in mouse corneal epithelial cells under high osmotic pressure and the samples for microarray. Meanwhile, mouse corneal epithelial cell lines (MCECs), transfected siRNA of NONMMUT047964.2 under high osmotic pressure, shows a decrease in apoptosis rate and a decrease in expression of IL-1β and IL-6. The experimental results show that the NONMMUT047964.2-miR-671-5p-Egr-1 axis may regulate the inflammation and promote the apoptosis of corneal epithelial cells under hypertonic condition.

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As for the tourism development of cultural community in ethnic area, the coexistence(symbiosis) of both the local tourism and local culture is not only connected with the relevant agents of them but also the relation of the relevant agents themselves. This is because harmonious coexistence(symbiosis) of the various agents is the elementary precondition of the ethnic cultural community tourism development. Symbiosis Theory was chosen as the theoretical perspective in this thesis, and Tu Nationality Hometown Park of Huzhu was selected as the study area. Then, symbiotic relationships and patterns of the various tourism agents were put into quantitative analysis. The findings displayed as the following: firstly, the four agents’ mutual characteristic symbiotic degree δM belongs to(ϵ) [0.719, 1.390], which illustrates that there exists the symbiotic relation between the every two agents of the four mutual agents and the symbiotic relations are totally positive symbiosis; secondly, the symbiotic coefficient 9m belongs to(ϵ) [0.34, 0.66], which approaches to 0.5 except the symbiotic coefficient of G-R(0.34). This illustrates that the mutual incidence of the other agents is basically the same except G-R; thirdly, the symbiotic relevancy of each agent approaches to one another, the symbiotic relevancy§m belongs to(e) [0.88, 1.08] concretely as R=E>G>T for the perspective of the tourism subject(tourists); lastly, the mutual behavioral patterns of the four agents of Tu Nationality Hometown Park of Huzhu belong to the patterns of positive reciprocal symbiosis(the positive symbiotic pattern of “residents-leading, enterprises-boosting” (REGT).