Objective: The present study was designed to study and compare the antibacterial and antioxidant activity of methanol extracts of Amomum subulatum Roxb. seeds collected from two different regions of Nepal.Methods: Antioxidant activity was determined by 1, 1-diphenyl-2-picrylhydrazyl assay and Antibacterial activity was measured by agar well-diffusion method. Results:Results showed that both sample extracts had interesting antibacterial activity against all test microorganisms and had remarkable radical scavenging potential to be used as an antioxidant. Sample B (i.e., collected from high altitude Taplejung district) exerted comparative quit higher antimicrobial and antioxidant activity than the Sample A (i.e., is collected from low altitude Panchthar district). However, the standard antibacterial drug Tetracycline exhibits superior activity than the extracts. Conclusions:The results suggest that both samples (i.e., seed extract of A. subulatum Roxb.) possess almost similar moderate antibacterial and antioxidant activity in comparison to standards which justifies the traditional use of this plant.