Background: CYP24A1 is the principal enzyme involved in the catabolism of 1,25(OH) 2 D 3 . Results: The SWI/SNF complex and PRMT5 converge at the transcriptional level to control 1,25(OH) 2 D 3 -induced Cyp24a1 gene expression. Conclusion: PRMT5-mediated repression represents a novel mechanism of negative regulation of Cyp24a1. Significance: Our study reveals key factors involved in the regulation of 1,25(OH) 2 D 3 catabolism and therefore in the control of calcium homeostasis.