Malaria is still a health problem in the world. Five species can infect humans, namely Plasmodium falciparum, Plasmodium vivax, Plasmodium malaria, Plasmodium ovale, and Plasmodium knowlesi.Four species are considered true parasites of humans, while P. knowlesi is still considered a zoonotic malaria. Among these species, P. falciparum and P. vivax are the most prevalent worldwide, in which the most common complications of severe malaria occur in P. falciparum infection. 1 The most frequent clinical manifestation of malaria is fever or recent history of fever. However, because many diseases also have fever as primary clinical manifestation even in the endemic area, accurate laboratory parameter is crucial. False-positive will lead to improper use of antimalarial therapy, and obviously, underdiagnosed cases cause an increase in morbidity, mortality, and antimalaria resistance. 2 The most frequently used method for early detection of malaria infection and remains the gold standard for laboratory confirmation of malaria is microscopic because it is easy to use and cheap. However, there are still many weaknesses in its application. 3

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