Background: Dental caries is the most common multifactorial infectious disease worldwide which referes to the process of destruction tooth hard tissue caused by bacterial by-products in the form of acids through carbohydrate fermentation. Lactobacillus acidophilus is one of the main cariogenic bacteria that causes caries by forming biofilms with the help ofglucosyltransferase (GTF) enzymes. Calcium hydroxide has limited antibacterial effects. Nano brown anchovy contains fluor as the active compound which has the potential as an antibacterial alternative agent. Purpose: To explain the inhibition of the combination of nano Stolephorus insularis and calcium hydroxide on the activity of the glucosyltransferase enzyme of Lactobacillus acidophilus bacteria. Methods: This study used a combination of nano Stolephorus insularis 3.125% with calcium hydroxide as a treatment and aquades as a control. The GTF enzyme was obtained from the supernatant centrifuged by Lactobacillus acidophilus in BHIB. The activity of the GTF enzyme was considered by calculating the fructose levels using High Performance Liquid Chromatography (HPLC) in a certain formula.Results: Decreased levels of fructose was obtained in the treatment group. From the results of Mann- Whitney data analysis, there were significant difference in the study groups. Conclusion: the combination of nano Stolephorus insularis with calcium hydroxide is effective to inhibit the glucosyltransferase enzyme activity of Lactobacillus acidophilus bacteria.

Background: The need for dental esthetics is very important in recent years. Fulfilling aesthetic demands with both non-invasive and minimally invasive techniques can maintain tissue continuity. A 38 year old female patient came to the clinic with an esthetic complaint of maxillary anterior teeth. The following case report describes a step by step complex aesthetic treatment of a patient with multiple caries. Appealing aesthetic results will restore the patient's smile through restorative treatment in dentistry. Purpose: The purpose of this case report is to explain the management of complex aesthetic treatment of patients with multiple caries Case: A 38-year-old female patient complained her upper front teeth is turning black. The patient complained that her teeth had hurt ± 3 months ago and sometimes felt sore. The patient wants her teeth to be treated because she feels less confident when she smiles and wants to fix these teeth. The  history  of  treatment on the tooth in question have had root canal treatment and been filled since 8 years ago. The tooth has had braces (orthodontic) treatment and was removed ± 7 years ago. The  patient  stated  that  she  had  no systemic health disorder. Case management : The treatment of this complex case were done using root canal treatment, endodontic retreatment, direct and indirect veneer, dowel, and crown. Conclusion: complex aesthetic treatment of patients with multiple were done aiming for good aesthetic result