Human being has varieties of facial configuration as similar to that of human dentition. Teeth present in the oral cavity having different types of characteristics with in respect to their external and internal anatomical and morphological configurations. This variability of root canal morphology affects the diagnosis, root canal treatment success rate and prognosis that have been always a challenge for a clinician.  Before starting an endodontic therapy, the dental professional should have a sound knowledge about root canal configuration. Vertucci’s type II root canal configuration in single-rooted mandibular second molar is the rarest case. The aim of this case series is to present the management of case of mandibular second molars with the rarest combination of single root and Vertucci’s type II canal configuration.