We report the infection efficiency and genetic diversity of three different viruses, cnidium yellowing vein virus (CnVYV), cnidium virus X (CnVX), cucumber mosaic virus (CMV), which are associated with yellowing and mosaic symptoms in Cnidium officinale plants in Iwate and Hokkaido, Japan. The rate of viral infection is extremely high and we found that all collected samples were infected with CnVYV. Phylogenetic analysis using coat protein (CP) sequences suggested that these three viral species had infected before or just after the cnidium plants were introduced in Japan.

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