The purpose of this research were: (1) to produce an appropriate micro-teaching video for the student teachers of Teacher Professionalism Training Program of Automotive Engineering Education, (2) to find out their responses to the micro-teaching video, and (3) to find out the effectiveness of the micro-teaching video in teaching the students. This research employed ADDIE model consisting of five stages namely analysis, design, development, implementation and evaluation. The product of this research was validated by the experts of media and materials in automotive engineering field. The product was tested on the students of Teacher Professionalism Training Program of Automotive Engineering Education Department. The research data were collected using questionnaire and observation sheet, and were analyzed through quantitative descriptive analysis. The results showed that: (1) micro-teaching video as learning media was classified into ‘very good’ level with the mean score 4.89 by the media expert, and also categorized into ‘very good’ with the mean score 4.67 by the materials expert, (2) the student teachers’ responses to the video during the try-out were classified into ‘very good’ level with the mean score 4.20, and (3) micro-teaching video as learning media was assumed to effectively improve the student teachers’ teaching skills. There was a significant improvement on the student teachers’ practice score, from 77.26 in the first practice to 83.59 in the second practice. In conclusion, micro-teaching video is an appropriate and effective learning medium for student teachers in Teacher Professionalism Training Program.