PurposeTo assess the role of asymptomatic genital tract inflammation in normozoospermic infertile men undergoing intracytoplasmic sperm injection (ICSI) and its outcome. Patients and methodsIn all, 70 normozoospermic male partners of infertile couples that underwent ICSI, were subjected to history taking, clinical examination, semen analysis, seminal staining for peroxidase, and estimation of seminal elastase. ResultsSeminal peroxidase staining failed to detect all cases of inflammation detected by the seminal elastase assay. The mean paternal age demonstrated significant increase in cases with elevated seminal elastase compared with cases with normal levels. There was nonsignificant difference in sperm count, sperm total motility, sperm morphology, and ICSI outcome (fertilization rate, good embryo rate, pregnancy rate, or abortion) in asymptomatic genital inflammation with/without elevated seminal elastase levels. ConclusionEstimation of seminal elastase is more accurate in detecting asymptomatic male genital tract inflammation compared with peroxidase stain. However, routine determination of seminal elastase in asymptomatic normozoospermic infertile men undergoing ICSI is of limited value.