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The Ammi visnaga seeds oil constituents of methyl ester derivatives of fatty acids were analyzed using gas chromatography coupled with mass spectrometer. The results obtained contain saturated as well as unsaturated fatty acids of A. visnaga seeds oils. A total of 15 different components were identified and quantified. Methyl ester of oleic acid was found in high concentration (13.30%) among the identified analytes of interest. In addition, methyl ester of linoleic acid (11.39%), palmitic acid (7.88%) and stearic acid (1.88%) were found. Concentration of the rest of identified fatty acids analytes were less than 1%. Thus, from the results it is apparent that the therapeutic efficacy of the plant may be due to the presence of high percentage of valuable analytes concentrations detected in the fatty acid of A. visnaga. The reason for the consumption in the pharmaceuticals as well as its wide applications in the prevention of dental carries, for various skin diseases and different infections.