This study is aimed to determine polymorphism of the Prolactin gene (PRL|PstI) in Sikumbang Jonti ducks using PCR-RFLP (Polymerase Chain Reaction–Restriction Fragment Length Polymorphism) method. This study used 56 Sikumbang Jonti ducks whose blood samples were taken. Gene amplification used a pair of primers forward 5' TGC AAA CCA TAA AAG AAA AGA 3' and reverse 5' CAA TGA AAA GTG GCA AAG CAA 3', which resulted in a 400 bp fragment in exon 5 of the Prolactin (PRL) gene. The amplification product was restricted using the PstI enzyme, which recognizes the truncation site (5' G↓ACGTC 3'). From 56 samples of Sikumbang Jonti ducks identified, just one genotype was found, homozygous (-/-) with only one allele (-). Analysis of the restriction product in Sikumbang Jonti ducks obtained a uniform genetic variation of PRL|PstI (monomorphic) with an allele frequency (-) of 100%.

Penelitian ini bertujuan untuk mengidentifikasi keragaman sifat kualitatif ayam burgo ayam hutan merah (Gallus gallus gallus), dan ayam kampung (Gallus gallus domesticus). Jumlah ayam ayam menggunakan adalah 74 ekor yang terdiri atas ayam burgo 47 ekor, ayam hutan merah (Gallus gallus gallus) 9 ekor, dan ayam kampung (Gallus gallus domestica)18 ekor yang dikoleksi di Bengkulu Selatan, Kota Bengkulu, Bengkulu Utara, dan Muko-muko, Provinsi Bengkulu. Karakteristik sifat kualitatif yang diamati adalah warna bulu, corak bulu, pola bulu, kerlip bulu, warna shank, tipe jengger, warna cuping, dan warna mata. Data sifat kualitatif berupa frekuensi fenotipe, frekuensi gen, nilai heterozigositas, nilai introgresi, dan gen asli. Ayam burgo dan ayam hutan merah memiliki keragaman yang rendah pada sifat kualitatifnya, sedangkan ayam kampung memiliki keragaman yang tinggi pada sifat kualitatifnya. Berdasarkan hasil penelitian, ayam burgo perlu dipertahankan dan dikembangkan sebagai sumber daya genetik ayam lokal Indonesia di Bengkulu.Kata kunci: Ayam burgo, ayam hutan merah, ayam kampung, sifat kualitatif

This study aims to identify the quantitative and qualitative performance of native chickens in The Mother breeding farm. This study used 50 male native chickens and 50 female native chickens aged 7 months. Variables observed included body hair pattern color, comb shape, comb color, shank color, eye color, beak color, body weight, chest circumference, shank length, shank circumference, body length, pubic bone length to chest, pubic bone distance and length wing span. Data were analyzed using descriptive analysis. The results of this study indicate that the body color of the male is Columbian (100%), while the female is solid black (62%), columbian (22%) and speckled or dotted (16%). The male cockscomb is pea (86%) and single (14%), while the female is pea (70%), walnut (20%) and single (10%). The color of the male comb is red (100%), while the female is red (98%) and black (2%). The shank color of males is yellow (74%), black (14%) and white (12%), females are black (74%), white (16%) and yellow (10%). Male eye color is orange (76%) and yellow (24%), while females are orange (66%), yellow (20%) and brown (14%). The color of the male's beak is yellow (54%), black and yellow (38%) and white (8%), while the female's beak is black and yellow (82%), yellow (10%) and white (8%). The results of the quantitative characteristics of the study showed that the male body weight was 2.22 ± 2.02 kg and the female was 1.71 ± 0.25 kg, the male chest circumference was 33.9 ± 3.68 cm and the female was 30.67 ± 2.08 cm, the length male shank 9.61 ± 0.89 cm and female 27 ± 0.59 cm, male shank circumference 5.52 ± 0.65 cm and female 4.4 ± 0.5 cm, male body length 20.27 ± 1.17 cm and female 20.40 ± 2.27 cm, the length of the pubic bone to the chest of the female is 7.87 ± 1.06 cm, the distance between the pubic bones is 2.8 ± 0.49 cm, the length of the male wing span is 23.28 ±2.18 cm and females 16.61±2.44 cm. The conclusion of this study is that the quantitative and qualitative characteristics of free-range chickens on The Mother farm are diverse and selection can be made. Keywords: Domestication chicken, Selection, Quality breeds, Lampung Province

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Growth Hormone Receptor (GHR) gene play important roles in duck performances due to their crucialfunctions in growth and has been considered as a candidate gene for growth traits. This reasearch wasaimed to indification polymorphism of Growth Hormon Receptor (GHR) gene in Sikumbang Janti duckand this study used 68 blood duck. Polymerase chain reaction (PCR) and sequencing analysis wereconducted to detect SNP existence in exon 10 of GHR gene. The result of this study showed that wasfound two SNP at g.1112 A>G and g.1304 C>T with transision mutation in exon 10 GHR gene. Genotypesfound in the g.1112 A>G were AA and GG with types of Allele A and G. Genotypes found in the g.1304T>C the were TT and CC with types of Allele T and C. Polymorphism of GHR gene were polymorphicand Population of Sikumbang Janti duck in Hardy-Weinberg equilibrium. It could be concluded thatGHR gene has potential to can be marker genetic in Sikumbang Janti ducks.