Chlorosubstitued3-Aroylchromone were prepared by refluxing of 1-(2-hydroxy-5-chlorophenyl)-3-(2 'furyl)-1, 3-propanedione and glutaraldehyde in ethanol and piperidine..The newly synthesized Chlorosubstitued 3-Aroylchromone were characterized on the basis of elemental analysis and spectroscopic data of IR,NMR. The titled compound were evaluated for their antibacterial activity against species such as Escherichia coli, Staphylococcus aureaus, Bacillus subtilis and Pseudomons aeruginosa