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Preparative scale purification of PEG-protein conjugates by centrifugal precipitation chromatography is discussed. By utilizing the transport of ammonium sulfate across a membrane and the centrifugal force, an ammonium sulfate gradient is formed and used for differential precipitation of PEG-protein conjugates. Native proteins can be separated from PEG-protein conjugates by using this technique.

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remained active after being subjected to both shear stress and precipitation in centrifugal precipitation chromatography. As a result, it can be concluded that centrifugal precipitation chromatography should be friendly to most proteins under the appropriate operating conditions.