I-Adamantanamine (amantadine) causes a selective, reproducible, dose-related inhibition of influenza infections in tissue culture, chick embryos, and mice. The compound is not virucidal and appears to act by interfering with the penetration of the host cell by the virus. In influenza infections of mice, greatest efficacy occurs with treatment at the time of infection; however, there is significant antiviral activity with treatment delayed up to 72 hours after infection. Virus inhibition is not complete and survivors are immune to a challenge infection with the original infecting virus.

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A new method for the rapid and precise determination of chlorogenic acid in green and roasted coffee is based upon the absorption of chlorogenic acid at 324 millimicrons.The chlorogenic acid is extracted from flaked coffee samples with water, and for green coffee the ultraviolet absorption is measured directly on the extract.

61amounts of non-protein nitrogen may be expected to affect the correlation of biuret protein with Kjeldahl protein (total N ~5 . 7 ) . In order to investigate the importance of this effect a series of wheat samples were milled on a Buhler laboratory mill, and Kjeldahl protein, biuret protein and non-protein nitrogen analyses carried out on the flour, the parent wheat and the bran removed during milling. The non-protein nitrogen content of the whole wheat was almost constant a t 3.5-4.5y0 of the total (Kjeldahl) nitrogen. The mean proportions of non-protein nitrogen present in the flour and bran were 2.1% and 123% respectively, showing that the removal of the bran layers during milling had simultaneously removed much of the non-protein nitrogen present in the original grain.For all practical purposes it was concluded that the non-protein nitrogen present in wheat and flour is unlikely t o give rise t o any significant differences between biuret and Kjeldahl protein contents of these materials. ConclusionsThe biuret reaction has been adapted to the rapid colorimetric estimation of protein in wheat and flour. The value of the method lies in the analysis of material for the benefit of cereal breeding institutions, flour mills and grain silos. Economically, the biuret method possesses tremendous advantages over the Kjeldahl process, the cost ratio of chemicals alone being approximately I : 40 in favour of the former, whilst the initial plant required is less expensive and less specialised. Technically the number of biuret analyses which can be carried out over a given period of time exceeds double the number of Kjeldahl determinations, are probably less susceptible to error, and do not entail the handling of large volumes of highly corrosive chemicals.Extracts of ox-muscle were investigated with a wew to identifying the reducing substsnces present which are responsible for the rapid browning cf these extracts at room temperaturc.Sugar phosphates were found to play an important part and the reactions of these phosphates with amino-acids in extracts and in model systems were followed.The development of colour and flavour in an extract, in a synthetic extract, and in model systems were investigated and found t o be a result of the Maillard reaction.