Thyroid gland is the largest endocrine gland supplied by the superior, inferior thyroid arteries and occasionally by thyroid ima artery. Proper identification of thyroid gland vessels is very important in order to avoid major complications during and after neck surgeries. The objective of this study was to assess variations on arterial supply of thyroid gland and its clinical significance in human cadavers in selected universities of north Ethiopia.A descriptive survey study design was employed to assess the variations on arterial supply of thyroid gland in human cadavers. Sixteen formalin fixed cadavers were used during routine dissection for the medical teaching. Variations in morphological character observed in the arterial supply of thyroid gland was noted, recorded and photographed.In the present study it was observed that, the site of the origin of superior thyroid artery (STA) was evaluated as it arose from external carotid artery in 84.4%, from carotid bifurcation in 9.375% and from common carotid artery in 6.25% the cases. Regarding to the number of STA was observed that single in all specimens. In all specimens, the origin of ITA was from the thyrocervical trunk.The anatomic characteristics and variation of the Thyroid arteries shows the most common site of origin of superior thyroid artery is from external carotid artery, whereas Inferior thyroid artery arisen from thyrocervical trunk and thyroid ima artery is not found.