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We report here the isolation of the cDNA for pea chloroplast SecA. Pea SecA encodes a polypeptide of 1,011 amino acids and shows high sequence similarity with cyanobacterial SecA. Pea SecA was synthesized as a larger precursor and was imported into isolated chloroplasts in vitro. The purified pea SecA, which was expressed in Escherichia coli cells, stimulated the in vitro import of the 33 kDa protein of the oxygenevolving complex into thylakoids. These results indicate that higher plant chloroplasts contain a bacterial-type SecA proteindependent system for the intraorganellar protein transport into thylakoids.

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