The capacity of sulfur-reducing bacteria Desulfuromonas acetoxidans Geobacter sp. CB35 and Desulfuromusa sp. CB30 and green photosynthesizing bacteria Chlorobium limicola their growth in wastewater of industrial and municipal origin. The strains of exoelectrogens, which are characterized by resistance to heavy metal ions, were isolated from the man-made Yavorivske lake located in the Lviv Oblast in Ukraine (D. acetoxidans Ch. limicola Geobacter sp. CB 30 and Desulfuromusa sp. CB 35). Bacteria D. acetoxidans fuel cell (MFC) with the application of D. acetoxidans W/m 2 and the reduction of chemical oxygen demand of wastewater was 99%. The new approach to improving the MFC performance was investigated. It includes a combination of phototrophic microorganisms Ch. limicola and heterotrophic microorganisms, which reduce the content of nitrates, nitrites, ammonia, sulfates, sulfites, hydrogen sulfide, while simultaneously generating electric current.