The study aimed to evaluate the prediction of ovulation time by three different methods body temperature, Dramineski estrus detection apparatus, and observation of the mature follicle daily by ultrasonography in mare's estrus. Sixteen mature cycling mares were used during the breeding season in15 March and15 June of 2021, aged 5-6 years old, at the Nineveh Equestrian club in Mosul city, Iraq. Mares were examined daily by rectal palpation and ultrasound with a 5.5-7 MHz linear-array transducer probe to scan the ovaries and uterus. The mare's follicle diameter and body temperature were recorded daily in the Dramineski estrus detection apparatus to predict ovulation when the data was up to 400 degrees. The result showed that the body temperature was increased significantly from the 1 st day to the third day of estrus at P<0.05, Dramineski estrus exhibited s elevation on the fourth day showing high significant variation at the 1st day to the third day and fourth day of estrus at P<0.01. The ovulation was higher in left than right ovary was recorded 62.5%. 37.5%, respectively. There was an increase in the follicular diameter scores during the third and fourth days of estrus at P<0.01. In conclusion, the combination of Dramineski estrus detection and preovulatory follicle diameter recorded daily is the best method to predict the time of ovulation, mares with a follicle diameter larger than 40 mm predicted the ovulation it has been pregnancy rate up to 75% after natural breeding service for only one time.