Dietary fiber is one of value added products from cassava pulp, byproduct of cassava starch industry. It could be produced by enzymatic digestion in which the optimal condition was studied. The three independent variables are heating temperature (°C) after adding α-amylase concentration (%v/v) and amyloglucosidase concentration (%v/v) regarding of two responses solid percentage yield and neutral detergent fiber (NDF) in the digested sample. The experimental design was based on central composite design. The highest solid percentage yield and NDF of cassava byproduct could get from enzymatic digestion condition at heating temperature at 90 °C, α-amylase concentration of 0.3 % (v/v), and amyloglucosidase concentration of 0.5 % (v/v). For ultrasound-assisted preparation was investigated at the optimum condition of enzymatic digestion. Ultrasound condition was control power of 140% and a frequency of 40 kHz for 15 min. The ultrasound was applied at pre-treatment condition (before adding α-amylase, PU) collocated with intermediate condition (before adding amyloglucosidase, IU) higher NDF yield than optimal condition at 18.78%. In addition, this ultrasound assisted technique give the highest solid percentage.
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