This study examined the history and development of the first nursing school in Thailand. Data were collected using a historical methodology through a review of related literature, as well as interviews with senior nurses. All data were clustered and categorized by using content analysis and an historical lens. The results revealed that the School development was divided to three periods: the School development in 1896-1925 (the foundation of modern Thai nursing); 1926-1955 (the influence of American medical and nursing education); and 1956-1971 (the transfer of a hospital-based training to university-based nursing education). In the final period, there was recognition that was associated with the professionalization of nursing, as the School initiated a bachelor degree in nursing and became the Faculty of Nursing. Through this long development, the School has progressively assisted Thai nursing to become a strong profession with international standards and recognition. The results of this study add significantly to the history of nursing in Thailand, and help nurses internationally to appreciate the efforts that have continued to sustain their noble profession.