The N,N,N-tridentate ligand, 1,3-bis(2'-benzimidazolylimino)isoindoline (1)

No abstract

A fluorometric method for determining estradiol valerate in sesame oil or ethyl oleate was subjected to collaborative study. The method utilizes a nitromethane-Celite column to isolate and purify the steroid, which is then measured fluorometrically. Four commercial samples and 3 synthetic samples, varying in concentration from 4 to 20 mg/ml, were used in the study. The collaborative results were acceptable: Coefficients of variation ranged from 2.48 to 3.55%; average recoveries for the synthetics ranged from 98.6 to 102%. The method has been adopted as official first action.

A fluorometric procedure has been developed for the quantitative determination of estradiol valerate in sesame oil or ethyl oleate. An n-heptane solution of the steroid is purified, using a nitromethane partition column. The purified estradiol valerate is dissolved in alcohol and determined fluorometrically. The method is applicable to a variety of commercial preparations, including estradiol valerate-testosterone enanthate combinations. A total of 14 determinations on 5 different synthetic preparations gave an average recovery of 97.2%. It is recommended that this method be subjected to a collaborative study.

A rapid screening method has been developed for determining methyl mercury in tuna and swordfish. Fish tissue is blended with acidic KBr solution to release methyl mercury, which is then extracted into methylene chloride. After cleanup by partitioning with cysteine, the methyl mercury is extracted into toluene and determined by gas-liquid chromatography. The proposed method compares favorably with the official AOAC atomic absorption method.