ABSTRACT:The kale is a brassica, this vegetable presents importance on nutritional and economic patterns. The present study aimed to evaluate the effect of different substrates, trays and protected environment in the formation of kale seedlings. The experiment was conducted in two greenhouses located at the State University of Mato Grosso do Sul -MS. The first environment consisted of an agricultural greenhouse with polyethylene film cover, while the second was an agricultural nursery with monofilament screen and mesh to offer 50% of shading. In each protected environment were used 72 and 128 cells tray and six different compositions of substrates with organic material based on cassava branches (CB) and cattle manure (CM): 1) 100% CM; 2) 20% CM + 80% CB; 3) 40% CB + 60% CM; 4) 60% CB + 40% CM; 5) 80% CB + 20% and CM; 6) 100% CB. The seedlings were evaluated according to the parameters: dry mass of seedlings, stem diameter and plant height. The agricultural nursery was the most suitable for the formation of kale seedlings, with 72 cells tray and the substrate composed of organic matter with 20% of cassava branches and 80% of cattle manure.