Oil palm is an oil crop that produces the highest oil yield throughout the year. Repeated application of fertilizer in oil palm orchard results in severe salinity of planting areas. Therefore, the objective of this study was to determine growth, physiochemistry and re-growth of calli as effected by sodium chloride (NaCl). The calli were cultured in liquid oil palm culture medium (OPCM) supplemented with different concentrations of NaCl (0, 50, 100, 150, 200, 300 and 400 mM), 0.1 mg/l dicamba and 200 mg/l ascorbic acid for 4, 8 and 12 weeks. The results showed that increase in concentrations of NaCl and exposure times decreased growth rate of cells in suspension culture. The concentration of NaCl which inhibited growth rate at 50 % (IC50) was 166.73 mM after culture for 12 weeks. Increase in concentrations of NaCl and exposure times increased electrolyte leakage (EL), changing of electrical conductivity (EC) medium and proline content. NaCl at concentration of 400 mM gave the highest EL, changing of EC medium and proline content at 82.22 %, 3.77 mS/cm and 64.93 µg/gFW, respectively. Over 200 mM NaCl oil palm cells cannot survive. After transfer treated callus clumps to OPCM without NaCl for 8 weeks, the clumps treated in 50 mM NaCl gave the highest fresh weight at 416.5 mg while 100 mM gave the highest average number of nodules per tube at 84 nodules. This result suggests that cells of oil palm in vitro could not stand to NaCl at concentration higher than 200 mM.