In humans, errors in meiotic chromosome segregation that produce aneuploid gametes increase dramatically as women age, a phenomenon termed the "maternal age effect." During meiosis, cohesion between sister chromatids keeps recombinant homologs physically attached and premature loss of cohesion can lead to missegregation of homologs during meiosis I. A growing body of evidence suggests that meiotic cohesion deteriorates as oocytes age and contributes to the maternal age effect. One hallmark of aging cells is an increase in oxidative damage caused by reactive oxygen species (ROS). Therefore, increased oxidative damage in older oocytes may be one of the factors that leads to premature loss of cohesion and segregation errors. To test this hypothesis, we used an RNAi strategy to induce oxidative stress in Drosophila oocytes and measured the fidelity of chromosome segregation during meiosis. Knockdown of either the cytoplasmic or mitochondrial ROS scavenger superoxide dismutase (SOD) caused a significant increase in segregation errors, and heterozygosity for an smc1 deletion enhanced this phenotype. FISH analysis indicated that SOD knockdown moderately increased the percentage of oocytes with arm cohesion defects. Consistent with premature loss of arm cohesion and destabilization of chiasmata, the frequency at which recombinant homologs missegregate during meiosis I is significantly greater in SOD knockdown oocytes than in controls. Together these results provide an in vivo demonstration that oxidative stress during meiotic prophase induces chromosome segregation errors and support the model that accelerated loss of cohesion in aging human oocytes is caused, at least in part, by oxidative damage. meiosis | maternal age effect | oxidative damage | reactive oxygen species | superoxide dismutase C hromosome segregation errors during female meiosis are the leading cause of birth defects and miscarriages in humans and their incidence increases dramatically with age (1). Over 90% of Down syndrome cases are the result of an extra copy of chromosome 21 inherited from the mother (2). Although the probability of a meiotic missegregation event is relatively low during a woman's twenties, by the time she reaches her early forties, she has a one in three chance of conceiving an aneuploid fetus (3). Work in the last decade has begun to shed light on the molecular mechanisms that underlie this phenomenon known as the "maternal age effect."Proper chromosome segregation during both mitosis and meiosis requires that physical linkages between sister chromatids (cohesion) be formed, maintained, and released in a regulated manner (4, 5). Sister chromatid cohesion, mediated by the evolutionarily conserved cohesin complex, is established during DNA replication. During meiosis, in addition to holding sister chromatids together, cohesion is required to maintain the physical association of recombinant homologs and is therefore essential for proper segregation during the first as well as the second meiotic division (6-8). Normally, a crossover ...

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TruePath facilitated successful crossing of infrainguinal CTOs following an unsuccessful guidewire recanalization, with significant improvement in symptoms and no complications.

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