Thomas Trichtinger scite author profile

The substrate specificity of 2'-deoxy-2'-substituted uridines and their 5'-phosphates towards thymidylate synthetase from Escherichia coli K12 was investigated. Besides the natural substrate 2'-deoxyuridine-5'-phosphate (dUMP), only 2'-deoxy-2'-fluorouridine-5'-phosphate (dUf IMP) was a substrate. The KM of dUfIMP is 11 times higher than that of dUMP, while the V values are virtually the same. It is concluded that the size of the 2'-su9gituent and not its polarity (and the concomitant conformational change) determines substrate specificity of thymidylate synthetase.

show abstract

The p-Nitrophenylethyl Group - An Universal Blocking Group in Nucleoside and Nucleotide Chemistry

Pfleiderer

Himmelsbach

Charubala

et al. 1985

Nucleosides and Nucleotides

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Thomas Trichtinger

The p-nitrophenylethyl (NPE) group

Synthesis of O6-p-nitrophenylethyl guanosine and 2′-deoxyguanosine derivatives

ChemInform Abstract: THE P‐NITROPHENYLETHYL (NPE) GROUP. A VERSATILE NEW BLOCKING GROUP FOR PHOSPHATE AND AGLYCONE PROTECTION IN NUCLEOSIDES AND NUCLEOTIDES

2′-Deoxy-2′-fluorouridine-5′-phosphate: an alternative substrate for thymidylate synthetase from Escherichia coli K12

The p-Nitrophenylethyl Group - An Universal Blocking Group in Nucleoside and Nucleotide Chemistry

Contact Info

Product

Resources

About