BackgroundImmunoglobulin A nephropathy (IgAN), membranous nephropathy (MN) and minimal-change disease (MCD) are three common types of glomerulonephritis in China. Pathological diagnosis based on renal biopsy is the criterion and the golden standard for diagnosing the sub-types of primary or secondary glomerulonephritis. Immunoglobulin and complements might be used in the differential diagnosis of glomerulonephritis without renal biopsies. However, the relationship between IF intensities of immune proteins and the corresponding serum levels remained unclear, and seldom studies combine histopathological examination results and blood tests together for a predictive purpose. This study was considered as a pilot study for integrating histopathological indicators into serum parameters for exploring the relationship of IF intensity and serum values of immunoglobulin and complement, and for screening and investigating effective indicators inIgAN, MN and MCD.MethodsRenal tissue immunofluorescence (IF) intensity grades and serum levels of immunoglobulin and complements (IgG, IgA, IgM, C3 and C4) were retrospectively analyzed in 236 cases with IgAN, MN or MCD. IF grades were grouped as negative (−), positive (+) or strong positive (++) with both high and low magnification of microscope. Other serum indicators such as urea nitrogen (BUN), creatinine (Crea) and estimated glomerular filtration rate (eGFR) were also evaluated among the groups.ResultsThere were difference in IgA, IgG and C3 IF intensity grades among IgAN, MN and MCD groups (p = 9.82E-43, 4.60E-39, 7.45E-15, respectively). Serum values of BUN, Crea, eGFR, IgG, IgA, IgM and C4 showed difference in three groups (BUN: p = 0.045, Crea: p = 3.45E-5, eGFR: p = 0.005, IgG: p = 1.68E-14, IgA: p = 9.14E-9, IgM: p = 0.014, C4: p = 0.026). eGFR had the trend to decrease with enhanced IgA IF positive grades (p = 8.99E-4); Crea had trends to decrease with both enhanced IgA and IgG IF intensity grades (p = 2.06E-6, 2.94E-5, respectively). In all subjects, serum IgA levels was inversely correlated with eGFR(r = − 0.216, p = 0.001) and correlated with Crea levels(r = 0.189, p = 0.004); serum IgG and Crea showed no correlation which were discordance with inverse correlation of IgG IF grade and Crea(r = 0.058,p = 0.379). IgG serum level was inverse correlated with its IF grades (p = 3.54E-5, p = 7.08E-6, respectively); C3 serum levels had significantly difference between Neg and positive (+) group (p = 0.0003). IgA serum level was positive correlated with its IF grades (Neg-(+): p = 0.0001; (+)-(++): p = 0.022; Neg-(++): p = 2.01E-10). After matching comparison among C3 groups, C3 Neg. group and C3 ++ group had difference (*p = 0.017). C4 had all negative IF expression in all pathological groups. In IgAN subjects, there were statistical differences of serum C3 levels between its pathological Neg and positive (+) group(p = 0.026), and serum IgA levels showed difference between IgA pathological positive(+) and (++)(p = 0.007). In MN subjects, sIgG levels showed difference between Ig...

Colorectal cancer (CRC) has become one of the top ten malignant tumors with a high incidence rate and mortality. Due to the lack of a good CRC screening program, most of the CRC patients are being transferred at the time of treatment. The conventional treatment cannot effectively improve the prognosis of CRC patients, and the target drugs can significantly prolong the overall survival of patients in the advanced stage. However, the use of single drug may lead to acquired drug resistance and various serious complications. Therefore, combined targeted drug therapy is the main alternative treatment with poor effect of single targeted drug therapy, which has important research significance for the treatment of CRC. Therefore, this study intends to culture CRC cell lines in vitro at the cell level and intervene with the GLP-1 receptor agonist liraglutide. The effects of liraglutide on the PI3K/Akt/mTOR signal pathway and CRC cell proliferation, cycle, migration, invasion, and apoptosis are explored by detecting cell proliferation, cycle, migration, invasion, and apoptosis and the expression of related mRNA and protein. The results showed that liraglutide, a GLP-1 receptor agonist, could block the CRC cell cycle, reduce cell proliferation, migration, and invasion and promote apoptosis by inhibiting the PI3K/Akt/mTOR signal pathway.

During operation, the acoustic signal of the drum shearer contains a wealth of information. The monitoring or diagnosis system based on acoustic signal has obvious advantages. However, the signal is challenging to extract and recognize. Therefore, this paper proposes an approach for acoustic signal processing of a shearer based on the parameter optimized variational mode decomposition (VMD) method and a clustering algorithm. First, the particle swarm optimization (PSO) algorithm searched for the best parameter combination of the VMD. According to the results, the approach determined the number of modes and penalty parameters for VMD. Then the improved VMD algorithm decomposed the acoustic signal. It selected the ideal component through the minimum envelope entropy. The PSO was designed to optimize the clustering analysis, and the minimum envelope entropy of the acoustic signal was regarded as the feature for classification. We then use a shearer simulation platform to collect the acoustic signal and use the approach proposed in this paper to process and classify the signal. The experimental results show that the approach proposed can effectively extract the features of the acoustic signal of the shearer. The recognition accuracy of the acoustic signal was high, which has practical application value.

When the shearer cuts coal or rock with different hardness, it will produce corresponding cutting state information. This paper develops a simulation cutting experiment system for the drum shearer based on similarity theory. It took the spiral cutting drum of a shearer as the research target and derived the principal similarity coefficients through the dimensional analysis method. Meanwhile, this paper designed the structure of the cutting power system and hydraulic system. Then, it chose a certain amount of coal powder as an aggregate, cement 325# as cementing material, sand, and water as auxiliary materials to prepare simulated coal samples. The paper adopted the orthogonal experiment method and used a proportion of cement, sand, and water as the influencing factors in designing a simulated coal sample preparation plan. In addition, it utilized the range analysis method to research the influence of various factors on the density and compressive strength of simulated coal samples. Finally, it conducted simulated coal sample cutting tests. The results show that the density of the simulated coal samples is between 1192.59 Kg/m3–1483.51 Kg/m3, and the compressive strength range reaches 0.16 MPa–3.94 MPa. The density of the simulated coal sample is related to the mass proportion of cement and sand. When the ratio gradually increases, the influence of sand increases. Furthermore, the compressive strength is linearly proportional to the proportion of cement. The self-designed simulation cutting experiment system could effectively carry out the relevant experiments and obtain the corresponding cutting condition signals through the sensors. There are differences in vibration signals generated by cutting different strength materials. Extracting the kurtosis value as the characteristic value can distinguish various cutting modes, which can provide a reliable experimental solution for the research of coal-rock identification.

When the shearer is cutting, the sound signal generated by the cutting drum crushing coal and rock contains a wealth of cutting status information. In order to effectively process the shearer cutting sound signal and accurately identify the cutting mode, this paper proposed a shearer cutting sound signal recognition method based on an improved complete ensemble empirical mode decomposition with adaptive noise (ICCEMDAN) and an improved grey wolf optimizer (IGWO) algorithm-optimized support vector machine (SVM). First, the approach applied ICEEMDAN to process the cutting sound signal and obtained several intrinsic mode function (IMF) components. It used the correlation coefficient to select the characteristic component. Meanwhile, this paper calculated the composite multi-scale permutation entropy (CMPE) of the characteristic components as the eigenvalue. Then, the method introduced a differential evolution algorithm and nonlinear convergence factor to improve the GWO algorithm. It used the improved GWO algorithm to realize the adaptive selection of SVM parameters and established a cutting sound signal recognition model. According to the proportioning plan, the paper made several simulation coal walls for cutting experiments and collected cutting sound signals for cutting pattern recognition. The experimental results show that the method proposed in this paper can effectively process the cutting sound signal of the shearer, and the average accuracy of the cutting pattern recognition model reached 97.67%.