Compared to conventional vehicles Hybrid Electric Vehicles (HEVs) provide fairly high fuel economy with lower emissions. To enhance HEV performance in terms of fuel economy and emissions, and ensure user satisfaction with driving performance, the need for simultaneous optimization for the main parameters of powertrain components and control system is inevitable. However, this problem is challenging due to the large amount of coupling design parameters, conflicting design objectives and nonlinear constraints. Considering the defect of the methods which convert multi-objective optimization problems into single-objective ones, a comprehensive methodology based on the non-dominated sorting genetic algorithms II (NSGA II) to achieve parameter optimization for powertrain components and control system simultaneously and successfully find the Pareto-optimal solutions set is presented in this paper. A case simulation is carried out and simulated by ADVISOR, The simulation results show that this method can produce many Pareto-optimal solutions and a satisfactory solution can be selected by decision-makers according to their requirements. The results demonstrate the effectiveness of the algorithms proposed in this paper.

Phytophthora genomes encode a myriad of Crinkler (CRN) effectors, some of which contain putative kinase domains. Little is known about the host targets of these kinase-domain-containing CRNs and their infection-promoting mechanisms. Here, we report the host target and functional mechanism of a conserved kinase CRN effector named CRN78 in a notorious oomycete pathogen, Phytophthora sojae. CRN78 promotes Phytophthora capsici infection in Nicotiana benthamiana and enhances P. sojae virulence on the host plant Glycine max by inhibiting plant H2O2 accumulation and immunity-related gene expression. Further investigation reveals that CRN78 interacts with PIP2-family aquaporin proteins including NbPIP2;2 from N. benthamiana and GmPIP2-13 from soybean on the plant plasma membrane, and membrane localization is necessary for virulence of CRN78. Next, CRN78 promotes phosphorylation of NbPIP2;2 or GmPIP2-13 using its kinase domain in vivo, leading to their subsequent protein degradation in a 26S-dependent pathway. Our data also demonstrates that NbPIP2;2 acts as a H2O2 transporter to positively regulate plant immunity and reactive oxygen species (ROS) accumulation. Phylogenetic analysis suggests that the phosphorylation sites of PIP2 proteins and the kinase domains of CRN78 homologs are highly conserved among higher plants and oomycete pathogens, respectively. Therefore, this study elucidates a conserved and novel pathway used by effector proteins to inhibit host cellular defenses by targeting and hijacking phosphorylation of plant aquaporin proteins.

No abstract

No abstract