The purposes of this study are to determine the effect of Zantac (Ranitidine) drug on sexual hormones (Testosterone and Prolactin), sperm head abnormality, and histopathological activity on albino male mice testes. Two doses of the drug were used: 1000 and 2000 mg/kg, in addition to a negative control group. Each group included four mice and the drug administrated orally as (0.1 ml) per day for 14 days, and then the mice were sacrificed on the day 15 for laboratory assessment. The result showed that the drug cause increase the percentage of sperm head abnormality which reaches to (48.3%) and (22.8%) for 2000 mg/kg and 1000 mg/kg respectively in comparison to control group (11.1%). The male sex hormones also affected by the drug and the level of testosterone hormone decrease to (1.07 ng/ml) in 2000 mg/kg and (3.42 ng/ml) in 1000 mg/kg, while the level of hormone in control group is (14.07 ng/ml). The prolactin hormone show increase in the level at dose 2000mg/kg it's (115 ng/ml) and in dose 1000 mg/kg the level its (43 ng/ml) these value very high compared with control group (36 ng/ml) due to the effect of the drug on the hormone. The histopathological examination shows damage in the seminal duct and changes in the wall of the seminiferous tube also the spermatogenesis well be affected by the drug in which is stop and some of seminal duct show no appearance of spermatogenesis and also cause depletion to the wall of the seminal duct.