Commercial automated vessel density measurements using OCT-A showed excellent repeatability in healthy individuals. Although repeatability will also need to be established in the setting of disease, the level of reproducibility should be useful for assessing the significance of differences in capillary density over time or under different conditions.

In corneas with guttae, both center and flex-center methods can reliably determine ECD. Although current practice recommends the center method when at least 100 cells can be counted, our study suggests that the center method can provide a reliable ECD value when there are ≥30 contiguous countable cells in a central cornea endothelial image. The flex-center method is recommended when <30 contiguous cells are identified.