Degradation of bisphenol A (BPA, 0.5 L, 30 mg L ) was studied by photoFenton treatment, while Fenton reagents were variables. The efficiency of the degradation process was evaluated by the reduction of total organic carbon (TOC), the biochemical oxygen demand (BOD), and toxicity. For toxicity analysis, bacterial methods were found infeasible, but the in vitro assay of VERO cells culture was successfully applied. Experiments according to a 2 design of experiments (DOE) with star points and three center points for statistical validity allowed selecting those process conditions (Fe(II) and H O load) that maximized the process performance. Photo-Fenton process effectively eliminated BPA and partly degraded its by-products (residual TOC <15 %) under substoichiometric H O dose (100.62 mg L ) and at least 4 mg L Fe(II), after a 90-min treatment. All