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BackgroundIn previous studies, the Mx Gene has been demonstrated to confer positive anti viral responses in chicken. The amino acid variation of Asn (allele A) at position 631 was specific to positive antiviral Mx/resistant, while, that of Ser (allele G) was specific to negative Mx/susceptible. This research was aimed at selecting one of the native chicken breeds which was found out to be resistant to avian influenza using molecular technique. The selected breed will then be used as the base population to improve native chicken breed in Indonesia.MethodsMarker Assisted Selection (MAS) method was used in this research to accelerate the selection process, since the disease resistance had low heritability value. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique used to select the genotype of Mx++, Mx+- and Mx-- that corresponded to the positive antiviral activity (Mx++), or those which had positive or negative activity (Mx+-) and negative antiviral activity (Mx--). There were 200 native hens and 40 cocks used in this experiment. Allele frequency of Mx Gene was calculated. The productivity indicators such as age at first laying, egg weight and hen weight at first laying and egg production were also measured. The chicken that had Mx++ and Mx+- genotypes, were selected to produce offspring.ResultsResult showed that the frequency of the resistant allele (Mx+) was 65% and 60% in laying hens and in cocks, respectively, while the frequency of the susceptible allele (Mx-) was 35% and 40% in hens and cocks, resepctively. Age, egg weight and hen weight at first laying and egg production for susceptible genotype were slightly better than for the resistant genotype which were 172,41 VS 178,81 days; 33,94 VS 32,84 g; 1450 VS 1439 g and 54,32 VS 48,30 %, respectively.

NRAMP-1 and iNOS genes were reported to be associated with a defense mechanism against bacteria and virus infections. This study aimed to identify NRAMP-1 and iNOS genes polymorphisms and their associations with the defense mechanisms against Salmonella enteritidis and Newcastle Disease (ND) in SenSi-1 Agrinak chicken. The present study used a total number of 172 SenSi-1 Agrinak chicken. Identifications of NRAMP-1 and iNOS genes polymorphisms were performed by PCR-RFLP method. NRAMP-1 and iNOS genotypes were associated with immunoglobulin Y (IgY) concentration, specific antibodies against S. enteritidis and ND using General Linear Model (GLM). Immunity characteristics were further grouped into high, medium, and low categories. NRAMP-1|SacI exon 11 and iNOS|AluI intron 24 in SenSi-1 Agrinak chickens were polymorphic. TC genotype has a higher immune response to infectious agents compared to TT and CC genotypes. The frequency of C allele was higher than the T allele in the concentration of immunoglobulin Y (IgY), antibodies titers against S enteritidis and ND. The TC genotype of NRAMP-1 gene was significantly associated with ND antibody titers, and the TT genotype of iNOS was significantly associated with S. enteritidis specific antibody. NRAMP-1 and iNOS genes can be used as potential candidate genes for immune traits in SenSi-1 Agrinak chickens.

ABSTRAKHidayat C, Iskandar S, Sartika T , Wardhani T. 2016. Respon pertumbuhan galur ayam lokal terseleksi terhadap ransum dengan kadar energi metabolis dan protein berbeda. JITV 21(3): 174-181. DOI: http://dx.doi.org/10. 14334/jitv.v21i3.1397 Penelitian ini bertujuan untuk mengetahui respon pertumbuhan galur ayam lokal hasil seleksi terhadap ransum dengan kadar energi metabolis dan protein ransum berbeda.. Tiga kelompok anak ayam hasil perkawinan galur ayam lokal terseleksi, yaitu Line 1 (♂KUB dengan ♀KUB), Line 2 (♂SenSi dengan ♀KUB) dan Line 3 (♂Gaok dengan ♀KUB), diberi tiga ransum perlakuan berbeda, yang terdiri dari ransum Diet 1 (2800 kkal ME/ kg dengan 17,81% protein kasar), Diet 2, (2950 kkal ME/ kg dengan 18,61% protein kasar) dan Diet 3 (3100 kkal ME/ kg dengan 19,25% protein kasar). Percobaan ini menggunakan rancangan percobaan faktorial 3x3. Setiap kombinasi perlakuan diulang sebanyak 7 kali dengan jumlah anak ayam umur sehari (DOC) sebanyak 5 ekor/ulangan, yang dipelihara sampai dengan umur 10 minggu. Hasil penelitian menunjukkan bahwa galur Line 2 (♂SenSi x ♀KUB) menunjukkan bobot hidup umur 10 minggu yang lebih tinggi (P<0,05), feed conversion ratio (FCR) yang rendah dan European Production Efficiency Factor (EPEF) yang tinggi, dibandingkan dengan kedua galur lainnya. Ransum yang optimum untuk galur Line 2 adalah ransum Diet 2, maka diambil kesimpulan bahwai galur ayam hasil perkawinan ♂SenSi dengan ♀KUB berpotensi untuk dimanfaatkan sebagai ayam lokal tipe pedaging komersial di Indonesia dengan menggunakan ransum optimum yang mempunyai kadar energi 2950 kcal ME/kg dengan 18,61% protein kasar. The aim of doing this experiment was to observe the growth response of improved native breeds of chicken under diets differed in energy and protein content. Three groups of improved native breeds, obtained from mating of ♂KUB to ♀KUB (Line 1), of ♂SenSi to ♀KUB (Line 2) and of ♂Gaok x ♀KUB (Line 3), were subjected to three experimental diets, differed in metabolizable energy (ME) and crude protein (CP) content. The experimental diets consisted of Diet 1 (2,800 kcal ME/ kg with 17,81% CP), Diet 2 (2,950 kcal ME/ kg with 18,61% CP) and Diet 3 (3,100 kcal ME/ kg with 19,25% CP). The experiment was designed as factorial 3 x 3 with 7 replications of each treatment combination, consisted of 5 birds per treatment combination. The chickens were raised up to 10 weeks of age. Results of the experiment showed that Line 2 (♂SenSi mated to ♀KUB) had highest body weight at 10 weeks of age (P<0.05), lowest feed conversion ratio (FCR) and highest European Production Efficiency Factor (EPEF), compared to other two lines. The appropriate diet for Line 2 was Diet 2. It could be concluded the crossbred line that was resulted from crossing of ♂SenSi to ♀KUB (Line 2), had potential to be used as improved native chicken for the industry in Indonesia supported by appropriate diet containing 2,950 kcal ME/kg with 18.61% crude protein.

Walik chicken having curly body feathers (frizzle). Adult Walik chickens are quite resistant to weather changes. However, Walik chickens have a rather limited population, existing only in certain regions, and are, therefore, rare local chickens that need to be further explored (Sartika et al., 2016). In addition to local chickens, Kampung Unggul Balitbangtan (KUB) chickens, which are the result of six generations of selection using female